Abstract
The preparation and modification of ethylene glycol dimethacrylate/2-hydroxyethyl methacrylate copolymers [poly (EGDMA- co-HEMA)] were carried out to study their properties on Immobilized Metal Ion Affinity Chromatography (IMAC). For that purpose, the adsorption–desorption assays of a phosphoprotein, ovalbumin (OVA) on different ion-containing adsorbents were investigated. The metal ion-complexing agent used was iminodiacetic acid (IDA) and the metal ions chelated were Al 3+ or Fe 3+. The efficiency on both modification reactions and OVA retention was analysed taking into account the pore volumes ( V p) and specific surface area ( S s) values in macropores zones and equilibrium volume-swelling ratio ( q v) from base matrices and final ion-containing supports. In general, Fe 3+-containing products resulted more efficient adsorbents in the OVA retention and although the adsorption assays on Fe 3+ or Al 3+-containing supports were fast, the complete and faster OVA desorption was reached when Al 3+-containing matrix was used.
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