Surface Modification of PHBV Nanofibrous Mat by Laminin Protein and Its Cellular Study

Abstract

Nanofibers have improved the performance of biomaterials, and can be considered effective. In this study, PHBV nanofibers were designed and then modified and crosslinked by oxygen plasma and laminin. The samples were evaluated by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscope (SEM), contact angle, mechanical analyses, and cell culture. ATR-FTIR structural analysis showed the presence of functional groups on the nanofibrous surfaces. The SEM images showed the average size of nanofibers to be about 100 nm for the samples. The 70° difference was obtained in the contact angle analysis, obtained for the laminin-crosslinked nanofibrous mat than the unmodified nanofibrous mat. Cellular investigation showed better adhesion and cell growth and proliferation of laminin-crosslinked nanofibrous samples than other samples. The bioavailability of PHBV fibers with covalently attached laminin was found to be identical to that of PHBV fibers with physically adsorbed laminin, indicating that covalent attachment of protein is a suitable method for enhancing the biocompatibility of tissue engineering scaffolds.