Abstract
An unmet need exists for the development of next-generation multifunctional nanocomposite materials for biomedical applications, particularly in the field of cardiovascular regenerative biology. Herein, we describe the preparation and characterization of a novel polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) nanocomposite polymer with covalently attached anti-CD34 antibodies to enhance capture of circulating endothelial progenitor cells (EPC). This material may be used as a new coating for bare metal stents used after balloon angioplasty to improve re-endothelialization. Biophysical characterization techniques were used to assess POSS-PCU and its subsequent functionalization with anti-CD34 antibodies. Results indicated successful covalent attachment of anti-CD34 antibodies on the surface of POSS-PCU leading to an increased propensity for EPC capture, whilst maintaining in vitro biocompatibility and hemocompatibility. POSS-PCU has already been used in 3 first-in-man studies, as a bypass graft, lacrimal duct and a bioartificial trachea. We therefore postulate that its superior biocompatibility and unique biophysical properties would render it an ideal candidate for coating medical devices, with stents as a prime example. Taken together, anti-CD34 functionalized POSS-PCU could form the basis of a nano-inspired polymer platform for the next generation stent coatings.
Highlights
Cardiovascular disease is the number one cause of death in the world, and it is estimated that by 2030, 23 million people will succumb annually to cardiovascular-related diseases [1]
Detection of amine on antibody-functionalized POSS-PCU via OPA assay The OPA assay revealed a higher fluorescence signals from POSS-PCU-CD34 compared to POSS-PCU
Fourier transform infrared (FTIR) studies were consistent with previous studies on POSS-PCU [30], which showed that these surface modifications did not alter the chemical integrity of POSS-PCU
Summary
Cardiovascular disease is the number one cause of death in the world, and it is estimated that by 2030, 23 million people will succumb annually to cardiovascular-related diseases [1]. Atherosclerosis is a subset of cardiovascular disease, and is characterized by a build up of fatty deposits in coronary arteries Left untreated, these blocked polymer-matrix platforms, and surface immobilized biomolecules to mitigate the risks of ISR and ST. POSS-PCU is a novel nanocomposite polymer, capable of functioning as a component of artificial organs [6], coatings for nanoparticles [7], and a platform on which bioactive molecules can be attached [8] It has been used in three different first-in-man studies as a lacrimal duct [6], as a bypass graft [9], and as the world’s first synthetic trachea [10]
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