Abstract
Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR) sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis. These operations are all performed under flow condition in the fluidic system, and have the advantages of easy implementation, less time consuming, and low cost, because the reagents and devices used in the operations are routinely applied in most laboratories. In this study, the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.
Highlights
Surface plasmon resonance (SPR) sensors play important roles in specic molecular interaction analysis owing to the features of high sensitivity, label free, and real-time monitoring.[1]
We describe an improved surface functionalization approach using polydopaminelm for SPR biosensors, where the polydopaminelm is prepared by circulating the dopamine aqueous solution through the sensing surface
The specic absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G (IgG) in the bu®er is measured by this system to demonstrate that this surface functionalization method can be applied for biomolecular interaction analysis
Summary
Surface plasmon resonance (SPR) sensors play important roles in specic molecular interaction analysis owing to the features of high sensitivity, label free, and real-time monitoring.[1]. The polydopaminelm, which is a great binding media for surface modication on the inorganic and organic surfaces,[9] is pointed to be an economical and convenient solution for rapid surface functionalization.[10] The reported surface modication method for label free biosensors is usually operated by immersing the sensor chip in the dopamine aqueous solution to coat the polydopaminelm on the sensing surface using dopamine self-polymerization.[11,12] The polydopaminelms are coated on the sensing surface of SPR chip and the other surface due to good adhesion of dopamine, and the polydopaminelm on the surfaces of SPR chips apart from the sensing surface should be removed to avoid the adverse effect on measurement before it is attached to the experimental system.[13] But the dopamine is sensitive to the light and air,[14] so the removing process of polydopaminelm, which inevitably makes the modicationlms exposed to the environment, has negative e®ect on the quality of surface modication and complicates the operations. The specic absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G (IgG) in the bu®er is measured to demonstrate the feasibility of proposed method for the surface functionalization of SPR biosensors
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