Abstract
Electrochemical biosensors have been widely applied to elucidate various information on pharmaceutically important neurotransmitters such as dopamine (DA). However, the biofouling of electrodes has been a critical issue for electrochemical measurements performed in physiological environments. In this study, highly biocompatible polymer brush made of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) was employed to modify carbon-based sensors to prevent biofouling. The PMPC brushes with thicknesses ranging from 15 to 30 nm on top of glassy carbon surfaces successfully suppressed protein adsorption in serum environments, and the polymer modification remained stable for at least 8 h in such conditions. The PMPC-modified glassy carbon electrode (GCE) achieved a limit of detection (LOD) as low as 1.8 μM for DA, as determined from cyclic voltammetry (CV), which exhibited better performance than the bovine serum albumin (BSA)-coated GCE with a LOD of 3.9 μM. The results from this study open new avenues for drug discovery and pharmacokinetics by enabling carbon-based biosensors to accurately and sensitively detect pharmaceuticals in protein-rich environments for an extensive period of time.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call