Abstract
In this study, copper nanoparticles (CuNPs) were synthesized using the extract of Opuntia ficus-Indica and Geranium as a reducing agent. By transmission electron microscopy, the morphology of the nanoparticles was determined to be mostly spherical, with a particle size about 3–10 nm. The UV–Vis spectra displayed absorption bands between 525 and 550 nm, associated with the surface plasmon resonance in the CuNPs. The various particle sizes obtained were tested as SERS substrate with the pyridine molecule. Antibacterial activity of copper nanoparticles against Escherichia coli gram-negative bacteria was studied. Bacteriological inhibition tests were performed on nutrient agar plates supplemented with different concentrations of copper nanoparticles. The copper nanoparticles with a concentration of 150 μg/mL and a size distribution around 6 nm showed the largest bactericidal effectiveness against E. coli.
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