SUPPRESSIVE EFFECTS OF WELSH ONION EXTRACTS ON MUCUS HYPER-PRODUCTION IN HUMAN AIRWAY CELLS

Akiko Takashima

doi:10.15436/2475-6245.16.011

Abstract

We investigated the suppressive effects of Welsh onion (Allium fistulosum L.) on the hyper-production of mucins in NCI-H292 human lung cancer cells. Periodic acid-Schiff staining showed that the 50% (v/v) ethanol extract of Welsh onion suppressed mucus glycoprotein production in cells, whereas the water and ethanol extracts did not. A real-time RTPCR analysis demonstrated that the 50% ethanol extract attenuated the gene expression of MUC2 and MUC5AC, which are major airway mucus components, in NCI-H292 cells stimulated with lipopolysaccharide (LPS). Furthermore, dot blot hybridization revealed that the extract at 100 μg/ml attenuated the hyper-production of the MUC5AC protein in LPS-stimulated NCI-H292 cells.

Highlights

Materials and MethodsApproximately 480,000 tons of Welsh onion (Allium fistulosum L.) are harvested each year in Japan
NCI-H292 human cancer cells produce mucus glycoproteins such as MUC5AC, and the protein levels of these glycoproteins are up-regulated by cytokines and chemical substances[8,9,10]
We examined the effects of Welsh onion extracts on the production of mucus glycoproteins such as MUC5AC using Periodic Acid-Schiff (PAS) staining and NCI-H292 cell growth

Summary

Introduction

Materials and MethodsApproximately 480,000 tons of Welsh onion (Allium fistulosum L.) are harvested each year in Japan. NCI-H292 human cancer cells produce mucus glycoproteins such as MUC5AC, and the protein levels of these glycoproteins are up-regulated by cytokines and chemical substances[8,9,10]. We previously established an assay system for intracellular and extracellular mucin proteins in NCI-H292 cells[13].

Results

Conclusion