Suppressive Effect of Auto-Polymerized Surgical Obturator Resin Incorporated with Vanillin on Microbial Biofilm

Abstract

Listen

Translate article

Surgical obturators are important oral prostheses given to patients after surgical treatment to minimize scar contracture and esthetic disfigurement that may affect patients’ functional appearance and psychological health. Poly-methyl methacrylate (PMMA) resin has been the material generally used for fabrication of surgical obturator. However, wearing of this resin appliance can raise a condition for microbial biofilm accumulation and infection of the patients due to the rough surfaces and internal porosities of the resin. Vanillin-incorporated heat-cured and auto-polymerized PMMA resin have been developed and demonstrated to have antimicrobial activity against several types of microorganisms. This study aimed to examine antimicrobial properties of vanillin-incorporated auto-polymerized surgical obturator resin on the biofilm formation of Streptococcus mutans and Candida glabrata. The auto-polymerized PMMA resin samples (Orthocryl® EQ, Germany) were prepared with vanillin incorporation (0.1% and 0.5% vanillin). All samples were coated with sterile saliva and incubated at 37 °C for 60 min. The suspensions of S. mutans ATCC 25715 and C. glabrata ATCC 15126 (107 colony forming unit/mL) were prepared using McFarland standard No.1. Microbial biofilm formation was done in 96-well plate at 37°C for 24 h. The total vital biofilm formation was evaluated by Cell Counting Kit WST-8 (Dojindo Molecular Technologies, USA) at 450 nm. All tests were done in triplicate on three separate circumstances. One-way ANOVA and Tukey’s test were applied for the statistical analysis. A significant decrease of S. mutans biofilm mass was noticed in 0.5% vanillin incorporated resin group compared with a control, resin without vanillin. The percentage of biofilm reduction was 40.8%. No different effect was observed in 0.1% vanillin group. In the case of C. glabrata, no significant biofilm reduction was examined among all groups. In conclusion, the adding of 0.5% vanillin to surgical obturator resin could significantly inhibit biofilm formation of S. mutans, however, the effect was not demonstrated on 0.1% vanillin. C. glabrata was observed to be resistant to vanillin. Using PMMA resin incorporated with 0.5% vanillin, an obturator with antimicrobial property can be applied to prevent dental caries and other systemic diseases in patients after surgical treatment.