Supported liquid extraction and ultra-high performance liquid chromatography-tandem mass spectrometry for the determination of urinary polycyclic aromatic hydrocarbon metabolites and their application for human biomonitoring

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are a class of highly lipophilic and ubiquitous, persistent organic pollutants with carcinogenic and mutagenic toxicities. They are a great public health concern, and avoiding exposure to them is a high priority. Human biomonitoring is critical for the evaluation of exposure levels to PAHs by the general population. In this work, we demonstrated the biomonitoring of eleven hydroxylated PAHs (OHPAHs) in urine samples from 226 volunteers from Guangzhou, and evaluated the health risks. The urinary PAH metabolites were released by enzymatic deconjugation, separated, and enriched by supported liquid extraction, and then quantified by ultra-high performance liquid chromatography-tandem mass spectrometry. The limit of quantification of the individual OHPAHs ranged from 10 ng/L to 40 ng/L, and satisfactory recoveries were obtained, ranging from 92.6% to 97.6%. The detection frequencies of the OHPAHs were 100%, and naphthalene metabolites were found at the highest concentrations with a geometric mean of 8.61 μg/L. The mean total OHPAH level in the urine samples of males (13.2 μg/L) was significantly higher than that of females (5.84 μg/L). Pearson correlation analyses indicated significant and positive correlations among urinary OHPAHs. The total estimated daily intake of PAHs was calculated, and a low health risk was obtained by evaluating their hazard quotients and hazard indexes.