Abstract

The aim of this study was to investigate the effects of dietary supplementation with a nonalcoholic red wine extract (RWE), including resveratrol and polyphenols, on insulin sensitivity and Sirt1 expression in nondiabetic humans. The present study was a single-arm, open-label and prospective study. Twelve subjects received supplementation with RWE, including 19.2 mg resveratrol and 136 mg polyphenols, daily for 8 weeks. After 8 weeks, metabolic parameters, including glucose/lipid metabolism and inflammatory markers, were evaluated. mRNA expression of Sirt1 was evaluated in isolated peripheral blood mononuclear cells (PBMNCs). Additionally, Sirt1 and phosphorylated AMP-activated kinase (p-AMPK) expression were evaluated in cultured human monocytes (THP-1 cells). Supplementation with RWE for 8 weeks decreased the homeostasis model assessment for insulin resistance (HOMA-IR), which indicates an increase in insulin sensitivity. Serum low-density lipoprotein-cholesterol (LDL-C), triglyceride (TG) and interleukin-6 (IL-6) were significantly decreased by RWE supplementation for 8 weeks. Additionally, Sirt1 mRNA expression in isolated PBMNCs was significantly increased after 8 weeks of RWE supplementation. Moreover, the rate of increase in Sirt1 expression was positively correlated with the rate of change in HOMA-IR. The administration of RWE increased Sirt1 and p-AMPK expression in cultured THP-1 cells. Supplementation with RWE improved metabolism, such as insulin sensitivity, lipid profile and inflammation, in humans. Additionally, RWE supplementation induced an increase in Sirt1 expression in PBMNCs, which may be associated with an improvement in insulin sensitivity.

Highlights

  • Metabolic derangement, including type 2 diabetes mellitus (T2DM), hypertension and dyslipidemia, which is based on insulin resistance, is closely related to the initiation and progression of cardiovascular disease (CVD) [1]

  • red wine extract (RWE) supplementation induced an increase in Sirt1 expression in peripheral blood mononuclear cells (PBMNCs), which may be associated with an improvement in insulin sensitivity

  • We investigated the effects of red wine polyphenols on glucose/lipid metabolism and Sirt1 expression in isolated peripheral blood mononuclear cells (PBMNCs) using red wine extract (RWE)

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Summary

Introduction

Metabolic derangement, including type 2 diabetes mellitus (T2DM), hypertension and dyslipidemia, which is based on insulin resistance, is closely related to the initiation and progression of cardiovascular disease (CVD) [1]. Maintaining metabolic health, including improving insulin sensitivity, is important to protect vascular tissues against metabolic-derangement-related cellular damage. Individual lifestyles, including dietary habits, affect metabolic and cardiovascular health. Appropriate consumption of red wine, 20–30 g/day as amount of alcohol, is thought to be part of a healthy lifestyle [2,3,4]. Previous epidemiological studies have shown an inverse association between dietary polyphenol consumption and mortality from CVD [5,6,7,8].

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