Supplementary Materials, Tables S1 and S2, Figures S1-S10 from Solid Tumors of Childhood Display Specific Serum microRNA Profiles

Abstract

<p>Supplementary Materials, Tables S1 and S2, Figures S1-S10. Supplementary Table S1. Number of over-expressed serum microRNAs in solid tumor types of childhood. Supplementary Table S2. Ten over-expressed serum microRNAs selected for the technical confirmation qRT-PCR study. Supplementary Table and Figure Legends, Supplementary References. Supplementary Figure S1. Quality control (QC) assessments in the initial qRT-PCR run. Supplementary Figure S2. Confirmation of suitability of the four serum QC housekeeping microRNAs for screening samples for the full discovery-phase qRT-PCR run. Supplementary Figure S3. QC assessments in the full discovery-phase qRT-PCR run. Supplementary Figure S4. Hemolysis assessment of the 53 serum samples in the initial QC and full discovery-phase qRT-PCR run. Supplementary Figure S5. Further QC assessment of the 53 serum samples in the full discovery-phase qRT-PCR run. Supplementary Figure S6. Top-ranking overlapping housekeeping microRNAs identified by NormFinder and geNorm algorithms, suitable for targeted serum qRT-PCR studies. Supplementary Figure S7. Comparison of expression of miR-122-3p and miR-122-5p in the 53 serum samples in the full discovery-phase qRT-PCR run. Supplementary Figure S8. Comparison of the average raw Ct values in the 21 serum samples common to both the full discovery-phase and technical confirmation qRT-PCR run. Supplementary Figure S9. QC assessment in the technical confirmation qRT-PCR run for all 25 serum samples used. Supplementary Figure S10. Serum microRNA expression in osteosarcoma.</p>