Supplementary Figure S1-9 from ROCK Inhibition Induces Terminal Adipocyte Differentiation and Suppresses Tumorigenesis in Chemoresistant Osteosarcoma Cells

Abstract

<p>Supplementary Fig. S1 Differentiation potential of AO and AX cells. Supplementary Fig. S2 High Intrinsic efflux capacity contributes to the chemoresistance of AO cells. Fig. S3 Immunofluorescence microscopy of FABP4 and PLIN1 expression in heterogeneous osteosarcoma cells. Supplementary Fig. S4 Effect of doxorubicin on the expression of PPARG, a master regulator of adipogenesis, in human osteosarcoma cell lines. Supplementary Fig. S5 AO-like cells expressing PPARG are present in human recurrent osteosarcoma tissue but not in parosteal osteosarcoma tissue. Supplementary Fig. S6 Treatment with fasudil alone induces terminal adipocyte differentiation in AO cells but not in AX cells. Supplementary Fig. S7 Effect of fasudil treatment on terminal adipocyte differentiation in AO cells. Supplementary Fig. S8 Treatment with fasudil alone induces growth suppression in human osteosarcoma cell lines. Supplementary Fig. S9 Effects of doxorubicin or fasudil treatment in AOT cells which have lost their adipogenic potential in AO cells.</p>