Supplementary Figure S10-19 from ROCK Inhibition Induces Terminal Adipocyte Differentiation and Suppresses Tumorigenesis in Chemoresistant Osteosarcoma Cells

Abstract

<p>Supplementary Fig. S10 Knockdown of either ROCK1 or ROCK2 alone induces terminal adipocyte differentiation and growth suppression of AO cells. Supplementary Fig. S11 Effect of chemical agents that induce depolymerization of the actin cytoskeleton on terminal adipocyte differentiation in AO cells. Supplementary Fig. S12 Immunohistochemical staining for MKL1 in an array of human osteosarcoma specimens. Supplementary Fig. S13 Effects of induction of a constitutively active mutant of MKL1 on the expression of MKL1 target genes in AO cells. Supplementary Fig. S14 Effects of fasudil treatment in mice injected with AO cells. Supplementary Fig. S15 Effect of fasudil treatment on terminal adipocyte differentiation in heterogeneous osteosarcoma cells. Supplementary Fig. S16 Effects of sequential treatment with doxorubicin and fasudil in heterogeneous osteosarcoma cells in vitro. Supplementary Fig. S17 Effects of sequential treatment with doxorubicin and fasudil in mice injected with heterogeneous osteosarcoma cells. Fig. S18 Osteogenic potential of AO and AX cells. Fig. S19 Effects of fasudil on the antiproliferative action of clinically administrated anticancer agents in AO cells.</p>