Abstract
Membrane curvature has recently emerged as a potent regulator of the targeted spatial organization (sorting) of proteins [1,2,3]. Many studies have shown this phenomenon by using purified components and simplified membrane models, such as vesicles or tubes. To date, few studies have been able to quantitatively report membrane curvature and its influence on protein recruitment in the context of a living cell. Here, we adapted a fluorescence microscopy-based method originally reported by Van Rheenen et al. [4] to extract a super-resolved topographic map of the basal membrane of a living cell. This allows for a direct visualization of membrane topography at ∼20 nm axial resolution, combined with quantitative curvature and protein density measurements. We envision that our approach will be instrumental in fully elucidating the contribution of membrane curvature to protein localization and function. 1. Hatzakis, N.S. et al.Nat. Chem. Biol. 5, 835-841 (2009). 2. Larsen, J.B. et al.Nat. Chem. Biol. 11, 192-194 (2015). 3. Rosholm, K.R. et al.Nat. Chem. Biol. 7 724-729 (2017). 4. Van Rheenen, J. & Jalink. K. Molecular biology of the cell, 9 3257-3267 (2002).
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