SUPEROXIDE–PRODUCING THERMOSTABLE ASSOCIATE FROM FLAXSEEDS, FLAXSEED CAKE AND SUNFLOWER SEEDS: ISOLATION, PURIFICATION, PROPERTIES AND MECHANISM OF SUPEROXIDE PRODUCTION

Abstract

he isoforms of superoxide (О2 — ) producing thermostable associate from flaxseeds (Linum usitatissimum), flaxseed cake (scale) and sunflower seeds (Helianthus annuus), which are formed between NADPH containing lipoprotein component (NLP) and NADPH oxidase (Nox): NLP-Nox, as prooxidative components, were isolated and purified for the first time. During isolation and purification of these associates its fractionation at pH 9,5, precipitation at pH 4,8 and ion-exchanging chromatography on cellulose DE52 were carried out. The specific amounts of these associates, its optical spectral indices, the immediate mechanism of О2 — production by these associates, stationary concentrations of produced О2 — in solution and gas phase (connected with oxygen) were determined (the arithmetic averages of the obtained values were determined). The specific contents of associates have the following sequence: sunflower seeds (32,6 mg/g), flaxseed cake (21,4 mg/g), flaxseeds (10,6 mg/g). The forms of optical absorption spectra of the water solutions of О2 — -producing associates from sunflower seeds, flaxseeds and flaxseed cake are practically similar. The stationary concentrations of produced О2 — in solution have the following sequence: sunflower seeds (32,5 mkM/mg), flaxseed cake (24,6 mkM/mg), flaxseeds (20,1 mkM/mg). After blowing of the aqueous solutions of above mentioned associates by oxygen monocomponent superoxide radicals (gas phase О2 — ), were produced and transferred with oxygen into glass tubes. The stationary concentrations of produced О2 — in gas phase 2 0 2 3 № 2 70 have the following sequence: sunflower seeds (25,2 mkM/mg), flaxseed cake (18,3 mkM/mg), flaxseeds (14,4 mkM/mg). These gas phase superoxide radicals can be used in experiments on laboratory animals. Inhalation of these radicals in certain concentrations can be a therapeutic method for respiratory diseases (still in experiment).