Superovulatory response, embryo recovery and progesterone secretion in Boran ( [formula omitted]) cows after treatment with either Pergovet or Pluset

Abstract

Boran ( Bos indicus ) cows (n = 24) were allocated, based on body weight, to be treated with either a human menopausal gonadotropin (Pergovet) at doses of 1050 or 1350 IU or a porcine gonadotropin (Pluset) at doses of 500 or 1000 IU. The cows were arranged in a balanced crossover design, and each cow was superovulated 3 consecutive times, which resulted in a total of 72 superovulations. Superovulatory treatment started on Day 10±1 of the estrous cycle, and cows were treated for 5 consecutive days. Estrus was induced with PGF 2α (2 ml, im) at 72 h after initiation of treatment. Cows were inseminated at 12 and 24 h after the onset of estrus using 2 doses of semen each from a Friesian bull. Daily blood samples were collected from the beginning of superovulatory treatment to the onset of estrus for determination of progesterone concentrations. The mean (±SEM) estrus response interval after PGF 2α and estrus duration were 33.1±4.1 and 9.3±0.5 h, respectively, and did not vary between treatment groups. Similarly, the mean numbers of palpable corpora lutea and follicular cysts were 7.8±0.7 and 3.0±0.4, with no treatment effect. The number of superovulations which yielded embryos/ova was 50 (69.4%) and was higher (P>0.05) in cows treated with Pergovet (75%) than with Pluset (63.9%). The mean number of embryos/ova recovered per superovulation was not affected by treatment and averaged 4.0±0.4, with a range from 3.2±0.9 to 4.6±1.0 in the 4 treatment groups. There was no treatment effect on the mean number of transferable embryos per superovulation, which averaged 3.1±0.3. The mean numbers of embryos/ova and transferable embryos per embryo yielding superovulation were 5.6±0.4 and 4.3±0.4, respectively. Return to natural estrus after the induced estrus averaged 43.3±6.1 d (range 37.2±11.8 to 56.4±12.2 d), with no apparent treatment effect. The mean numbers of embryos/ova, transferable embryos and interval to return to natural estrus did not vary between consecutive superovulations. Mean plasma progesterone concentrations dropped from 7.6±0.3 ng/ml on the first day of superovulatory treatment to 1.3±0.1 ng/ml on the day of induced estrus and did not differ between treatment groups and between cows which yielded different numbers of embryos/ova. Progesterone concentration on the day of estrus was negatively correlated with the number of embryos/ova (−0.29) and the number of transferable embryos (−0.26), suggesting that progesterone concentration on the day of induced estrus can help to predict embryo yield and quality in Boran cows. It was concluded that both Pergovet and Pluset can be used to superovulate Boran cows with no residual effect of repeated treatment on embryo yield and return to natural estrus. However, treatment with Pergovet tended to increase the proportion of cows which yielded embryos. Moreover, Pergovet given at 1050 IU and Pluset at 1000 IU resulted in the production of 19.4 and 39.0% more number of transferable embryos than at 1350 and 500 IU, respectively.