Abstract
POLYOMA and SV40 DNA can be extracted from productively infected cells in the form of protein-DNA complexes distinct from the virus1,2. In the presence of puromycin, association of newly-synthesized polyoma DNA with protein was found to be curtailed and the covalently closed circular DNA contained very few superhelical turns3. No evidence was found to support a mechanism whereby superhelical turns were introduced into polyoma DNA independently of replication. We report here similar effects of puromycin on the nature of protein-DNA complexes in SV40 virus infected cells. We demonstrate that superhelical turns can be introduced into SV40 DNA independently of replication and present information on the fate of modified protein-DNA complexes in terms of virus assembly.
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