Abstract
ABSTRACT The DNA in a eucaryotic nucleus is arranged into a series of supercoiled loops which are anchored at their bases to a nuclear skeleton or matrix. It can be shown that these supercoiled loops of DNA are mobile, as they appear to be reeled through the matrix during DNA replication. Using nuclease digestion, one can progressively cleave DNA from the loops, thereby isolating residual DNA that is close to the nuclear matrix anchorage sites. Several transcribed genes appear to be enriched in this residual DNA. The sequences examined are the genes coding for ribosomal RNA in rat liver cells, the SV40 genes in SV40 transformed mouse cells, the ovalbumin gene in hen oviduct cells, and the Alu sequence family in human lung cells. The results suggest that genes transcribed by all three mammalian RNA polymerases are oriented non-randomly with respect to supercoiled loop domains.
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