Abstract
Structured illumination microscopy (SIM) has emerged as an essential technique for three-dimensional (3D) and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM, from bespoke hardware and software development and the use of commercial instruments to biological applications. This special issue aims to recap recent developments as well as outline future trends. In addition to SIM, we cover related topics such as complementary super-resolution microscopy techniques, computational imaging, visualization and image processing methods.This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.
Highlights
Cite this article: Prakash K, Diederich B, Reichelt S, Heintzmann R, Schermelleh L. 2021 Super-resolution structured illumination microscopy: past, present and future
To date, there has not been a dedicated workshop or journal issue covering the various aspects of structured illumination microscopy (SIM), from bespoke hardware and software development and the use of commercial instruments to biological applications
Fluorescence light microscopy is a core technique in life sciences that has contributed to countless major discoveries
Summary
Fluorescence light microscopy is a core technique in life sciences that has contributed to countless major discoveries. In the past two decades, several super-resolution microscopy (SRM) approaches have been developed that overcome this barrier and push the spatial resolution to the 10–150 nm range, closing the gap to electron microscopy [2,3,4,5] These SRM techniques can be further divided into single-molecule localization microscopy (SMLM) [6] that includes techniques like photoactivatable localization microscopy (PALM) [7,8] and stochastic optical reconstruction microscopy (STORM) [9], stimulated emission depletion (STED) [10,11,12] microscopy, and structured illumination microscopy (SIM) [13,14]. The uniquely balanced combination of properties has made SIM remarkably successful for a wide range of biological applications promoting new discoveries [22, 33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48]
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