Abstract
In continuous culture set-up for sulfate-reducing bacteria a sulfide electrode (made from silver wire) is used to control the electron donor supply and the medium pump. The sulfied concentration of the medium is kept at a low level by continuosly flushing out H 2S and replacing it with CO 2. The pH is controlled automatically by regulating the CO 2 content of the gas mixture flushed through the medium. With the sulfide-controlled set-up sulfate-reducing bacteria can be grown in chemostat culture under electron donor as well as electron acceptor limitation. Furthermore, by continuously washing out the culture to a preselected residual sulfide concentration, cells can be grown in sulfidostat culture under non-limiting conditions at maximal growth rate. Growth yields of Desulfotmaculum orientis, when growth in this system with hydrogen as electron donor, were considerably higher than previously reported.
Published Version
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