Abstract
Sulfated derivatives of hyperbranched β-glucans from the edible mushroom Pleurotus tuber-regium (S-HBGs) were synthesized with varying degrees of substitution (DS) in the current study to evaluate their hypoglycemic and anti-glycation efficacy. Characterization analysis revealed that sulfation minimally affected the polymer's branched structure but reduced its molecular weight. Transmission electron microscopy (TEM) confirmed that the branched and interwoven chain conformation of S-HBGs remained largely intact despite the presence of sulfate groups. S-HBGs demonstrated reversible inhibition of α-glucosidase, with the inhibition mode transitioning from competitive to mixed-type and eventually to non-competitive as DS decreased from >0.70 to 0.55 and 0.27. Furthermore, S-HBGs with moderate or low DS effectively inhibited protein glycation and the formation of advanced glycation end products (AGEs). These findings advance the understanding of S-HBGs as novel hypoglycemic and anti-glycation agents, underscoring their potential as bioactive food components for managing diabetes and its complications.
Published Version
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