Abstract
Mono-, di- and trihexose sulfatides have been isolated from hog gastric mucosa lipids by the procedure involving column fractionations on DEAE-Sephadex, silicic acid and thin-layer chromatography. Desulfation of mono-, di- and trihexose sulfatides with methanolic HCl resulted in the formation of galactosylceramide, lactosylceramide and galactosyllactosylceramide, respectively. By partial acid hydrolysis, periodate oxidation and methylation analysis, the structures of these sulfatides were identified as: (SO 3H→ 3)-Gal-(1→ 1)-Cer,(SO 3H→ 3)-Gal-(1 → 4)-Glc-(1 → 1)-Cer and (SO 3H → 3)-Gal-(1 → 4)-Gal-(1 → 4)-Glc-(1 → 1)-Cer, respectively.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
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