Sulfated capsular polysaccharide from the marine bacterium Kangiella japonica inhibits T-47D cells growth in vitro

Abstract

Kangiella japonica KMM 3897 is a Gram-negative bacterium isolated from a coastal sea-water sample of the Sea of Japan. In this paper, the results about the structure and the antiproliferative effect on cancer cells of the capsular polysaccharide isolated from the Kangiella japonica KMM 3897 have been described. The carbohydrate polymer was isolated and purified by several separation techniques, and the structure was elucidated using chemical analysis and NMR spectroscopy. The following structure of the sulfated capsular polysaccharide, containing 2-amino-2-deoxy-D-mannuronic acid was established:→3)-α-D-GlcpNAc4,6S-(1→4)-β-D-ManpNA3Ac-(1→4)-β-D-GlcpNAc-(1→.The capsular polysaccharide exerted a selective antiproliferative effect and suppressed the colony formation of T-47D cells.