Abstract
Psychrobacter marincola KMM 277T is a psychrophilic Gram-negative bacterium that has been isolated from the internal tissues of an ascidian Polysyncraton sp. Here, we report the structure of the capsular polysaccharide from P. marincola KMM 277T and its effect on the viability and colony formation of human acute promyelocytic leukemia HL-60 cells. The polymer was purified by several separation methods, including ultracentrifugation and chromatographic procedures, and the structure was elucidated by means of chemical analysis, 1-D, and 2-D NMR spectroscopy techniques. It was found that the polysaccharide consists of branched hexasaccharide repeating units containing two 2-N-acetyl-2-deoxy-d-galacturonic acids, and one of each of 2-N-acetyl-2-deoxy-d-glucose, d-glucose, d-ribose, and 7-N-acetylamino-3,5,7,9-tetradeoxy-5-N-[(R)-2-hydroxypropanoylamino]- l-glycero-l-manno-non-2-ulosonic acid. To our knowledge, this is the first finding a pseudaminic acid decorated with lactic acid residue in polysaccharides. The biological analysis showed that the capsular polysaccharide significantly reduced the viability and colony formation of HL-60 cells. Taken together, our data indicate that the capsular polysaccharide from P. marincola KMM 277T is a promising substance for the study of its antitumor properties and the mechanism of action in the future.
Highlights
Many marine bacteria can produce extracellular polysaccharides (EPSs)
Bacterial EPSs usually occur in two forms: As capsular polysaccharides (CPSs), if they are associated with the cell surface, and medium-released polysaccharides (MRPs), if they are completely released in the environment [1]
The dried bacterial cells were subjected to extraction with around structure around P. marincola KMM 277 cells [13]
Summary
Bacterial EPSs usually occur in two forms: As capsular polysaccharides (CPSs), if they are associated with the cell surface, and medium-released polysaccharides (MRPs), if they are completely released in the environment [1] The presence of these biopolymers indicates their specific properties and functions that are beneficial to microorganisms; they play an essential role in protecting the bacterial cell from harsh environmental conditions (salinity, temperature, and the availability of nutrients), in surface adhesion (usually through the biofilms formation), intercellular signal transduction, and in resisting the host’s immune response. All these features help to survive and protect their producers from the complex marine environment [1,2].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
