Abstract
Sulfate metabolism in extracts of embryonic chick and calf cartilage and a human chondrosarcoma of the sternum of a 75-year-old patient have been compared. The chondrosarcoma was able to activate and transfer sulfate to acid mucopolysaccharide, at a level approaching that of the embryonic extracts, when comparison was made with adult human costal cartilage extracts which had feeble activity. CSA-A, 1 1 Abbreviations used: CSA-A and CSA-C, chondroitin sulfates A and C, respectively; PAPS, adenosine 3′-phosphate 5′-sulfatophosphate; APS, adenosine 5′-sulfatophosphate; ATP, adenosine triphosphate. incompletely sulfated, was the only mucopolysaccharide isolated from the myxomatous areas of the chondrosarcoma, but the presence of CSA-C was suggested by infrared spectral data. Keratosulfate was absent, and thus the mucopolysaccharide composition resembled that in newborn costal cartilage rather than the pattern of adult costal cartilage. Incorporation of sulfate-S 35 into acid mucopolysaccharide in vivo and in vitro gave a fraction isolated by paper chromatography, which was excluded from Sephadex G-50. The suggested presence of a minute S 35 subfraction, retained on Sephadex G-50 and therefore of mol. wt. < 8–10,000, was obtained. Sulfate-S 35 was also incorporated into a number of endogenous acceptors present in the cartilage extracts. Phenol sulfokinase was present in both avian and mammalian cartilage, but the avian cartilage extracts were unique in being able to sulfate phenolic steroids and it appears in this case that both simple phenols and the phenolic steroids are sulfated by the same enzyme.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call