Sulfate facilitates cadmium accumulation in leaves of Vicia faba L. at flowering stage

Abstract

Cadmium (Cd) is a toxic element, and is prevalent all over the world because of industrialization, mining, sewage sludge, or pesticide supply. Sulfur deficiency is also a frequent problem faced in agriculture. To date, information relating to effects of sulfate on Cd toxicity is still limited. To elucidate how sulfate affects Cd accumulation in faba bean, subcellular accumulation of Cd in leaves consisting of apoplastic washing fluid, symplastic fluid and the cell wall under deficient, sufficient and excess sulfate treatments were investigated in the present study. By using stable isotope of Cd (106Cd), we also traced Cd accumulation in young leaves at flowering stage from early and newly uptake of Cd in the same plants as affected by sulfate. We found that excess sulfate supply significantly increased newly uptake of Cd without affecting early uptake of Cd when compared with sufficient sulfate treatment, which resulted in enhanced total Cd in leaves by excess sulfate. Since newly uptake of Cd in leaves was from root uptake directly, we conclude that excess sulfate supply enhanced Cd originated from root uptake directly rather than re-translocation from old leaves, which is related to increased Cd accumulation in young leaves of faba bean. Subcellular analysis showed that the enhanced Cd by excess sulfate addition was a consequence of enhanced Cd in cell walls, while Cd accumulation in the apoplastic washing fluid and symplastic fluid were unchanged. The increased Cd by excess sulfate supply might be related to increased proportion of Cd speciation CdSO40 in the growth medium because of faster diffusion of CdSO40 than Cd2+. To test whether macronutrients, micronutrients, sulfate and non-protein thiol involved in cell wall-Cd accumulation as affected by sulfate, correlations of subcellular Cd with subcellular macronutrients, micronutrients, sulfate, and non-protein thiol were analyzed. We found that cell wall-Cd was negatively correlated with K and Ca concentrations, whereas cell wall-Cd was positively correlated with Cu and symplastic non-protein thiol concentrations. However, when compared with sufficient sulfate, excess sulfate decreased K concentration and increased symplastic non-protein thiol concentration without changing Ca and Cu concentrations. Based on those results, reduction of K concentration and enhancement of symplastic non-protein thiol concentration by sulfate supply might be a reason for increase of cell wall-Cd concentration. Taken together, increased Cd in cell walls of leaves by sulfate supply contributes to enhance Cd accumulation.