Sulfadiazine inhibits hydrogen production during sludge anaerobic fermentation by affecting pyruvate decarboxylation

Abstract

The overuse and random discharge of antibiotics can cause serious environmental pollution. Sludge acts as a repository for antibiotics, its anaerobic fermentation process will inevitably be affected. This study investigated the effects of a typical antibiotic contaminant, sulfadiazine (SDZ), on the anaerobic fermentation of sludge for hydrogen production. Results demonstrated that the production of hydrogen was significantly inhibited by SDZ, and the inhibition was enhanced with increasing SDZ content. Within 5 days, the cumulative amount of hydrogen with 500 mg SDZ/kg dry sludge was 8.5 mL, which was only 32.2% of that in the control (26.4 mL). Mechanistic investigation showed that the reduced hydrogen production when SDZ existed was mainly attributed to the suppression of pyruvate decarboxylation during the hydrogen production stage, and the diversity of microorganisms, especially the abundance of microorganisms and the activities of key enzymes closely related to hydrogen production were inhibited with SDZ, resulting in less hydrogen accumulation.