Sucrose concentration and volume of liquid medium on the in vitro growth and development of blackberry cv. Tupy in temporary immersion systems

Ricardo Antônio Ayub,Daurimar Mendes Da Silva,Tereza Cristina De Carvalho,Fernanda Grimaldi,Jessé Neves Dos Santos,Luiz Antônio Zanlorensi Junior

doi:10.1590/1413-7054201943007219

Ricardo Antônio Ayub, Daurimar Mendes Da Silva + Show 4 more

Open Access

https://doi.org/10.1590/1413-7054201943007219

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Abstract

ABSTRACT Micropropagation of small fruits such as blackberry has been employed due to the need to obtain plants with high phytosanitary quality. Bioreactor technology has been used to improve efficiency in seedling production. Thus, the objective of this work was to evaluate the best culture medium volume and sucrose concentration for blackberry micropropagation in a temporary immersion bioreactor. In vitro blackberry shoots were segmented containing two buds and an internode (1.0 cm) and placed into MS medium supplemented with inositol (0.1 g L-1), BAP (1 mg L-1) and sucrose (10, 20, 30 or 40 g L-1) at different medium volumes (150, 175 and 200 mL). The total length, number of leaves, number of shoots, and number of hyperhydric shoots were evaluated 56 days after start of the project. For blackberry development and propagation in a bioreactor system, the best results were shown at a medium volume of 175 ml and a sucrose concentration of 20 g L-1.

Highlights

The blackberry (Rubus spp.) belongs to the small fruit group, and its production in Brazil is concentrated in the South and Southeast regions
Using liquid medium enables the automation of the process, presenting advantages for reducing labour and production costs (Silva et al, 2007; Teixeira, 2011; Georgiev et al, 2014)
In the experiment evaluating the amount of culture medium, the number of shoots (Figure 2 A) per explant was 44% and 35% higher using 175 mL than using 150 and 200 mL, respectively

Summary

Introduction

The blackberry (Rubus spp.) belongs to the small fruit group, and its production in Brazil is concentrated in the South and Southeast regions. The conventional technique of micropropagation requires high labour, the use of a large quantity of containers (Teixeira, 2011) and semi-solid culture medium, which, in addition to sucrose, low light intensity and high relative humidity in the containers, can reduce the photosynthetic potential and stomatal functionality, generating a lower rate of multiplication and biomass, making the technique more expensive (Hazarika, 2006; Lemos, 2013) With this perspective, another option for in vitro plant development is the use of liquid medium, allowing greater contact of the medium with the explant and providing greater nutrient absorption (Rodrigues et al, 2006). The use of liquid medium should be optimized for each species and condition, since it is more propitious to water accumulation in the plant’s apoplast, which decreases gas exchange with the external environment and causes oxidative stress and, as a consequence of the plant morphology, generates a vitreous state (Rojas-Martínez; Visser; Klerk, 2010; Dries et al, 2013)

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