Sucrose concentration and blueberry plant density in temporary immersion systems (TIS)

Ricardo Antonio Ayub,Daurimar Mendes Da Silva,Isabela Leticia Pessenti,Jessé Neves Dos Santos,André Belmont Pereira

doi:10.1590/0100-29452021166

Ricardo Antonio Ayub, Daurimar Mendes Da Silva + Show 3 more

Open Access

https://doi.org/10.1590/0100-29452021166

Copy DOI

Abstract

Abstract The aim was to determine the ideal concentration of sucrose along with the number of plants per container in the face of micropropagation of blueberry under TIS. In the experiment conducted with sucrose concentration (0, 10, 20, e 30 g L-1), we utilized a WPM medium, taking into account 30 explants per container. For the experiment dealing with blueberry plant density, the same medium with 20 g L-1 of sucrose in conjunction with 30, 40, 50 and 60 explants per container was used. After 90 days from the installation of the trial, the following response variables were assessed herein: total length of the plant, number of shoots, length of the largest shoot, number of hyperhydric shoots, length of hyperhydric shoots, and number of healthy leaves. Under a sucrose concentration corresponding to 20 g L-1 the best possible results were obtained. Survival rates of 22 and 80% under the influence of both 10 and 20 g L-1 treatments were reported, pointing out that plants exposed to 20 g L-1 treatment were more amenable to acclimatization. Thus, for the micropropagation of blueberry under TIS the use of 20 g L-1 sucrose and 60 plants/container comes to being highly recommended to increase yield.

Highlights

Blueberry (Vaccinium ashei) is a shrub belonging to Ericaceae family, which is native from Europe and North America and featuring an eccentric flavor along with nutritional properties that cause the fruit to be highly valued all over the world, with 540 thousand tons produced in 2014 agricultural harvest (CANTUARIAS-AVILÉS et al 2014)
Throughout acclimatization, we observed that in view of variable air relative humidity thresholds the 10 g L-1 of sucrose treatment was conducive to a 22% survival, whereas blueberry plants exposed to the 20 g L-1 of sucrose treatment resulted in an 80% survival rate (Figure 7)
Our outcomes corroborate the findings reported by Debnath (2017), which highlighted that a survival rate ranging from 80 to 90% throughout acclimatization was obtained whenever plants were grown under temporary immersion systems (TIS) and stationary systems, respectively

Summary

Introduction

Blueberry (Vaccinium ashei) is a shrub belonging to Ericaceae family, which is native from Europe and North America and featuring an eccentric flavor along with nutritional properties that cause the fruit to be highly valued all over the world, with 540 thousand tons produced in 2014 agricultural harvest (CANTUARIAS-AVILÉS et al 2014). Since its introduction in Brazil, such a woody species has been expanding in terms of planted area; this increment is restricted to a low viability of seeds (PASQUALINI et al 2016). Apart from such a particular problem, further drawbacks related to low quality of plantlets, lack of phytosanitary warranty and delays in production might be circumvented by the micropropagation technique. For blueberry a remarkable alternative lies in the multiplication in bioreactors of temporary immersion systems (TIS), which differently from the conventional system makes use of a liquid medium that provides a more substantial contact between explant and medium in such a way as to better absorption of nutrients and ensure a maximization of crop growth and yield at a given site, apart from allowing for automation of the process by reducing costs plus labor (RODRIGUES et al 2006, SILVA et al 2007, TEIXEIRA 2011, GEORGIEV et al 2014, AYUB et al 2019)

Methods

Results

Conclusion