Subtypes of α1‐ and α2‐Adrenoceptors Mediating Noradrenergic Modulation of Spontaneous Inhibitory Postsynaptic Currents in the Hypothalamic Paraventricular Nucleus

Abstract

2-6 of the above paper contained a number of errors in the previously published version. The corrected figures are reproduced below. Blockade of the noradrenaline-induced spontaneous inhibitory postsynaptic current (sIPSC) frequency decrease by BRL44408, an α2A-AR antagonist, but not by prazosin, an α2B/2C-AR antagonist. (a) and (b) Time course histograms showing the effects of noradrenaline on sIPSC frequency of paraventricular nucleus (PVN) neurones in the presence of (a) BRL44408 (3 µM) or (b) prazosin (100 µM). (c,d) Current records of the same neurones at the times shown by the arrows in (a) and (b), respectively. (e,f) Mean effects of noradrenaline (30–100 µM) on sIPSC of types I and II PVN neurones in the presence of (e) BRL44408 (1–10 µM) or (f) prazosin (20–100 µM). BRL, BRL44408; Pra, prazosin. Values are presented as means ± SEM from six type I and five type II neurones for BRL44408 and from three type I and five type II PVN neurones for prazosin. Significantly different from the controls at *P < 0.05, **P < 0.01 and ***P < 0.001 by Student's t-test, respectively. Noradrenaline-induced increases in spontaneous inhibitory postsynaptic current (sIPSC) frequency in the presence of RS17053, an α1A-AR antagonist, or BMY7378, an α1D-AR antagonist in type II paraventricular nucleus (PVN) neurones. (a,b) Time-course histograms showing the noradrenaline-induced sIPSC frequency increase in the presence of (a) RS17053 (10 µM) or (b) BMY 7378 (2 µM). (c,d) Current records of the same neurones at the times shown by the arrows in (a) and (b), respectively. (e,f) Mean effects of noradrenaline in the presence of (e) RS17053 (10 µM, n = 5) or (f) BMY7378 (2–20 µM, n = 4). RS, RS17053; BMY, BMY7378. Significantly different from the control at *P < 0.05 by t-test (noradrenaline) or the Mann–Whitney U-test (BMY + noradrenaline), or **P < 0.01 by t-test. Concentration dependent block of noradrenaline-induced spontaneous inhibitory postsynaptic current (sIPSC) frequency increase by prazosin in type II paraventricular nucleus (PVN) neurones. (a) Time-course histogram showing the effect of noradrenaline on sIPSC frequency in the presence of prazosin at 0.1 and 1 µM. (b) Current records showing changes in synaptic activity at the times shown by the arrows in (a). (c) Mean effects of noradrenaline on sIPSC frequency the presence of prazosin at 0.1 µM (n = 5) and 1 µM (n = 6). *P < 0.05 by Student's t-test.