Abstract
The design of a full-scale bioprocess is typically based on parameters derived from smaller-scale experiments from a previous study. However, disagreements often occur at up-scaling of waste-to-energy processes due to the fluctuations of the substrate characteristics, etc. Therefore, once a commercial-scale waste digester has been built and operated, it is essential to test if the performance of the process agrees with its design value; during this process, fluctuations might occur in digesters operated at marginal organic loading rates. In this study, triplicate full-scale anaerobic digesters treating food waste were monitored for five months. The digesters, operated at the design feeding ratio, showed increasing volatile fatty acid (VFA) trends (per total alkalinity) due to a 30% higher chemical oxygen demand of the influent, than the design. The organic loading rate was adjusted on a daily basis until a stable performance was observed. Significant shifts of methanogen populations from Methanobacteriales to Methanomicrobiales and Methanosarcinales were observed during the stable operation period.
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