Submerged production of mycelium biomass and bioactive compounds from P. ostreatus in a controlled fermentation medium

Abstract

Pleurotus ostreatus is commonly consumed mushroom species around the world due to its unique taste, flavor, and presence of biologically active compounds. The current study investigated the biological activities of P. ostreatus in submerged cultivation. Cultivation factors such as pH, temperature, inoculum density, and cultivation time with different carbon and nitrogen sources were optimized to obtain a high yield of biomass and exopolysaccharides (EPSs). The maximum biomass and EPS obtained were 24.66 ± 0.2 g/L and 4.8 ± 0.1 g/L respectively. The protein content and total phenolic content (TPC) were achieved as 0.936 ± 0.005 g/L and 16.484 ± 0.06 mg GAE/g respectively at 12th day of cultivation. Antioxidant activity of P. ostreatus was obtained as 49.59 ± 1.09%. Under optimized conditions, the P. ostreatus was also cultivated in a rotary bed reactor (RBR). Furthermore, different kinetic models were studied to observe the growth behaviour of mycelium biomass. The best fit was obtained for the contois model, which gave the maximum specific growth rate (µmax, hr−1) of 0.0235 hr−1 and R2 value of 0.976.