Abstract
Animal waste fats were explored as a fermentation substrate for the production of high-value unsaturated single cell oil (SCO) using oleaginous fungi, Mucor circinelloides and Mortierella alpina. Both strains showed good growth and lipid accumulation when using animal fat as a single carbon source. The biomass concentration of 16.7 ± 2.2 gDCW/L and lipid content of 54.1%wt (of dry cell weight) were obtained for Mucor circinelloides in shake flask experiments, surpassing the biomass yield achieved in batch and fed-batch fermentation. In contrast, Mortierella alpina gave the highest biomass concentration (8.3 ± 0.3 gDCW/L) and lipid content (55.8%wt) in fed-batch fermentation. Fat grown Mortierella alpina was able to produce arachidonic acid (ARA), and the highest ARA content of 23.8%wt (of total lipid weight) was in fed-batch fermentation. Gamma-linolenic acid (GLA) was produced by both fungal strains. At the end of fed-batch fermentation, the GLA yields obtained for Mucor circinelloides and Mortierella alpina were 4.51%wt and 2.77%wt (of total lipid weight), respectively. This study demonstrates the production of unsaturated SCO-rich fungal biomass from animal fat by fermentation.
Highlights
The global production and consumption of meat is projected to rise progressively over the decade, largely driven by population and income growth [1]
The shake flask cultivations of M. circinelloides and M. alpina strains were performed by using glucose and animal fat rest materials as carbon source
M. circinelloides reached stationary phase after 42 h with a final lipid content of 50.1%wt, while M. alpina showed slower growth with the stationary phase observed after 120 h and a final lipid content of 54.6%wt (Figure 2)
Summary
The global production and consumption of meat is projected to rise progressively over the decade, largely driven by population and income growth [1] This demand for meat will result in the increased production of slaughterhouse by-products from the meat processing industry. Category 1 and 2 ABPs are not suitable for human and animal consumption, while category 3 by-products are of lowest risk and are fit for human consumption. Today they are not used directly for human food, but as animal feed and for commercial purposes [3,4,5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
