Abstract
The aim of this study was to produce single cell oil (SCO) from Lipomyces starkeyi ATCC 56304 using a biphasic fed-batch fermentation strategy. Fed-batch fermentations were performed to compare oil production using glucose, xylose, mixed-sugars (1:1 ratio of glucose:xylose), and a biphasic system supplying glucose for cell growth and xylose for oil production. The fermentation using xylose as the sole carbon source generated an oil yield of 0.16gg−1 and a productivity of 0.12gL−1h−1, two-times the oil yield (0.08gg−1) and productivity (0.06gL−1h−1) achieved with glucose. At 120h, the biphasic fermentation produced a greater oil productivity (0.13gL−1h−1) compared to the mixed-sugar fermentation (0.09gL−1h−1) but similar oil productivities (0.14gL−1h−1) were achieved for both at longer fermentation times. The biphasic fermentation generated a final oil yield of 0.17gg−1 and an oil content of 60.1% (w w−1), but oil productivity showed minor improvement at 0.14gL−1h−1 after 255h. Oleic acid accounted for at least 65% of the fatty acid content, suggesting that oil from L. starkeyi has potential for application in the biofuel and oleochemical industries.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.