Abstract
Abstract Background Hepatic fibrosis is a complex and dynamic process similar to “wound healing” that results in the progressive accumulation of connective tissue. We aimed to investigate the epigenetic control of liver fibrosis and Hippo pathway in human hepatic stellate cell (HSC) line. We examined the effect of Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor on the LX2 cell line. Material and methods 2.5 μM SAHA was treated to LX2 cell line for 2 days. Cell proliferation and apoptosis measurement were performed by Muse Cell Analyzer. Yes-Associated Protein/Transcrıptional Coactivator With Pdz-Binding Motif (YAP/TAZ) and alpha-smooth muscle actin (α-SMA) protein expression levels were measured by western blotting. Results In our study, we observed that the SAHA treatment reduced cell viability and induced apoptosis of LX2 cells statistically. We found that SAHA treatment decreased α-SMA, YAP and TAZ proteins levels statistically. Conclusion Decreased cell viability could be due to physiological, autophagical and also related to the apoptotical mechanisms. We thought that SAHA plays an important role in the creation of the fates of the LX2 cell line.
Highlights
Materials and methodsMany of the biological events are controlled by various posttranslational modifications that realize in the amino tails of histones [1]
YAP/TAZ proteins, which play a role in Hippo pathway, are thought to be new players as nucleolar mechanosensors during organ homeostasis and cancer formation [14]
In the Suberoylanilide hydroxamic acid (SAHA)-treated group, we found decreased cell viability compared to the control group
Summary
Many of the biological events are controlled by various posttranslational modifications that realize in the amino tails of histones [1]. Among these modifications, the most studied histone modification is acetylation. Histone acetyl transferase (HAT) and histone deacetylase (HDAC) enzymes are controlled by histone acetylation. It effects important molecular events [2]. Acetylation is a reversible phenomenon and HDAC enzymes have been the target of varios drugs with the implication that they effect important cellular events [5, 6]. We examined the effect of Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor on the LX2 cell line.
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