Subculture human skeletal muscle cells to produce the cells with different Culture medium compositions

Ying Balch

doi:10.31579/2693-4779/036

Abstract

This study aimed to subculture human skeletal muscle cells (HSkMC) using a culture medium with different compositions to determine the most efficient medium for the growth of the human skeletal muscle cells. The culture media was divided into three groups: Group1. An HSkMC growth medium. Group 2. An HSkMC growth medium + with 10% high glucose (GH). Group 3. An HSkMC growth medium + 10% fetal bovine serum (FBS). HSkMC from groups 1 to 3 gradually became round in shape and gathered in clusters. These changes differed between the groups. In group 3, the HSkMC clusters were more in numbers and gathered as significantly more prominent than in the other groups under the EVOS-Microscope shown. We concluded that by manipulating the composition of the culture medium, it is possible to induce HSkMC to promote the best growth.

Highlights

Evidence suggests that inflammation and apoptosis play a role in a skeletal muscle cell associated with diminished skeletal muscle cell function with healthy aging [1, 2,3,4,5]
In group 3, the human skeletal muscle cells (HSkMC) clusters were more in numbers and gathered as significantly more prominent than in the other groups under the EVOS-Microscope shown
HSkMC was capable of growing by manipulating the culture medium composition

Summary

Introduction

Evidence suggests that inflammation and apoptosis play a role in a skeletal muscle cell associated with diminished skeletal muscle cell function with healthy aging [1, 2,3,4,5]. Caspases-3 typically exists in an inactivated state in the cytoplasm, but it can be activated by proteolytic cleavage, and hetero-dimerization and activates pro-caspase-3 start the caspase cascade [8, 9]. Once the proteolytic cascade is switched on, it eventually cleaves and enables pro-caspase-3 to induce the inflammation to the loss of skeletal muscle [9]. This study aimed to subculture human skeletal muscle cells (HSkMC) using culture media with different compositions to determine the most efficient medium for the growth of the human skeletal muscle cells to ensure that the research experiment more efficient

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Conclusion