Abstract
The subcellular distribution of cytochrome oxidase (mitochondrial), NADH cytochrome c reductase (nuclei, mitochondrial, microsomal, soluble) and NADPH cytochrome c reductase (microsomal, soluble) marker activities were analysed biochemically in human inflamed and dilantin hyperplastic gingiva. Protein and enzyme distribution patterns were found to be similar in the inflamed and dilantin hyperplastic gingival subcellular fractions. Cytochrome oxidase was found primarily within the mitochondrial fraction, and NADPH cytochrome c reductase was found chiefly within the soluble fraction. NADH cytochrome c reductase distributed in each subcellular fraction, and was found within the nuclear, mitochondrial, microsomal and soluble fractions. Significantly greater activity (cytochrome oxidase, NADH cytochrome c reductase and NADPH cytochrome c reductase) was noted in mildly inflamed dilantin hyperplastic tissue than was observed in mildly to moderately inflamed gingiva. NADH cytochrome c reductase (mitochondrial) was significantly greater than cytochrome oxidase in both tissue fractions, and was rotenone, amytal and antimycin a, insensitive which suggests that the activity may be primarily associated with the outer mitochondrial membrane. The increased specific activities of the enzyme markers in dilantin gingiva may reflect metabolic activity associated with cell mitosis, proliferation, protein (collagen) synthesis and drug detoxification.
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