Abstract
The histochemical activities of succinate dehydrogenase, cytochrome oxidase, monoamine oxidase, NADH and NADPH cytochrome c reductases, three NAD-dependent dehydrogenases (glutamate, lactate, and malate) and one NADP-dependent dehydrogenase (glucose-6-phosphate) were determined in ovaries from 19 bitches of various ages representing different stages of the estrous cycle. The principal enzyme differences concerned those enzymes related to steroid metabolism (i.e., glucose-6-phosphate dehydrogenase and NADPH cytochrome c reductase) and were observed at different stages of the estrous cycle. The theca interna and enzymatically active stromal cells (EASC) possessed greatest activity during proestrus and estrus, whereas the corpus luteum showed strongest activity during midmetestrus. Medullary sex cords of the immature ovary possessed high activity of all of the coenzyme-dependent dehydrogenases. Subsurface epithelial structures (SES) of the mature ovary demonstrated enzyme activity similar to germinal epithelium and epithelium of the rete ovarii which appeared distinctly different from primary follicles. Oocytes in primary follicles possessed lactate and malate dehydrogenase and NADH cytochrome c reductase activity. Early follicular atresia was accompanied by increased glucose-6-phosphate dehydrogenase and NADPH cytochrome c reductase activity in granulosa cells.
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