Subcellular distribution of multiple forms of acid p-nitrophenyl phosphatase in rat kidney

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1. 1. Two forms of acid p-nitrophenyl phosphatase activity are present in rat kidney separable from subcellular fractions of renal cortical homogenates by gel filtration on Sephadex G-100. 2. 2. The lysosomal and the microsomal enzymes had molecular weight (mol. wt) of greater than 100,000 and had similar kinetic and inhibitory properties. 3. 3. In contrast, the soluble fraction contained not only this high molecular weight form but also a lower molecular weight form (mol. wt 20,000). 4. 4. This latter form differed in its response to inhibitors, had different K m value but had the same acid pH optimum.