Abstract
Subcellular fractionation of a complex proteome (cardiac tissue) allows for enrichment of a subset of low abundant proteins. This permits more in depth analysis of the proteome by reducing the complexity. Adult rat cardiac tissue was made ischemic by ligating the left anterior descending coronary artery in vivo for 1 hour with no reperfusion, and the healthy remote area was differentiated from the ischemic tissue by staining with Evans Blue dye after harvesting the heart. A series of differential centrifugation steps produced nuclear, mitochondrial, cytoplasmic, microsomal, and sarcomeric fractions of rat ischemic and remote healthy tissue.
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