Abstract
G A A b st ra ct s the effect of PHGG on stress mimicked corticotropin-releasing hormone (CRH) induced animal IBS model. Materials and methods. Six-week-old male Wistar rats were used in this study. Animals were allowed to acclimate to the environment for seven days before initiating the study. Under anesthesia, each rat was implanted stainless steel cannula placed towards the lateral cerebral ventricle. Then rats were divided into two groups of 8 animals each, (1) control group: diet without PHGG, (2) PHGG group: diet with 5% PHGG for seven days. After intracerebroventricular (icv) injection of CRH (10 μg / rat) or saline in each, the condition of stools was checked with a 2-hour fecal pellets output count. Three hours later, rats were sacrificed, and cecal contents were taken out to analyze the microbiota with terminal restriction fragment length polymorphism (T-RFLP). Results. The counts of stools were increased in CRH-treated animals compared to in saline-treated group animals with the control diet. However, in rats in PHGG group with icv of CRH, the numbers of stools were not increased. In PHGG group Clostridium subcluster XIVa, which thought to be products butyric acid and Bifidobacterium cluster were enlarged, but not in the control diet group, then after injection of CRH, Bifidobacterium cluster was decreased. Conclusions. PHGG improved CRH-induced bowel dysmotility and also modulated in the gut microbiota composition which were influenced by CRH administration. Our results indicate that braingut axis is deeply associated with gut microbiota in physiological mechanism in IBS. This study was supported by Japan Science and Technology Agency.
Published Version
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