Abstract
We report the detection and quantification of important ulcerative colitis drugs olsalazine (OLS) and sulfasalazine (SUL) by the spectrofluorometric method. The proposed method was optimized and validated by using the quenching effect on the acriflavine fluorescence. The method was applied on the detection and quantification of OLS and SUL under optimized conditions showing the calibration curves were linear (range: 1.0–10.0 µg ml−1), with correlation coefficients R2 of 0.9999 for both drugs. The limits of detection (LOD) and quantification (LOQ) were 53 and 104 ng ml−1 for the OLS and 160 and 315 ng ml−1 for the SUL. This method permitted the analysis of OLS and SUL in their pure and pharmaceutical forms. The proposed spectrofluorimetric method was also evaluated against ‘green’ criteria and all the experimental results make it an eco-friendly and safe method for the detection of OLS and SUL.
Highlights
Olsalazine (OLS) and sulfasalazine (SUL) are effective agents used for treating ulcerative colitis either alone or with corticosteroids and for maintaining remission [1]
The proposed method relies on quenching of the native acriflavine fluorescence by OLS and SUL in the buffered medium
Britton– Robinson buffer (BRB) has been investigated over the pH range of 3–10, and it was found that the optimum pH was 7 for both OLS and SUL
Summary
Olsalazine (OLS) and sulfasalazine (SUL) (figure 1) are effective agents used for treating ulcerative colitis either alone or with corticosteroids and for maintaining remission [1]. The investigated drugs have been evaluated in the literature by using spectrophotometric [10,11,12,13], spectrofluorimetric [14,15], HPLC [16,17,18] and electrochemical methods [19,20,21]. The study aim was to investigate a proposed method based on acriflavine quenching for rapid quantification of OLS and SUL. An aqueous solution of acriflavine (8 × 10–4 mol l−1; Sigma-Aldrich, Germany) consisting of 0.0208 g acriflavine in 100 ml distilled water was diluted to a concentration of 8 × 10−6 mol l−1. The standard solutions of both OLS and SUL were prepared by dissolving 10.0 mg of the drug in distilled water or methanol, respectively, in a 100 ml volumetric flask and diluted to volume with the respective solvent. Further dilutions were carried out to reach the studied concentration range
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