Studying the Expression of RUNX3 gene and Its CpG Island Methylation Status in bladder transitional Cell Carcinoma

Abstract

Objectives To detect hypermethylation status of the 5'CpG island locating in the promoter re - glon of RUNX3 gene and the expression level of their mRNA and protein in bladder transitional cell carcinoma (BTCC).Methods Using methylation - specific PCR(MSP) technique to detect methylation status of RUNX3 gene in BTCC,and to detect their mRNA and protein expression level by RT - PCR and Westem blot method.Re - suits The frequenee of Promoter methylation of RUNX3 gene Was 46.9%(15/32) in BTCC tissues,but it Was not found in normal bladder tissues.The frequenee of Promoter methylation wag increased with the increasing tumor grade and stage(P<O.01).In 15 patients with the aberrant Promoter methyiation,14 patients shown the loss ex - pression of RUNX3 gene(r=0.6472,P=0.012).The expression of RUNX3 mRNA and protein in the normal bladder tissues and BTCC tissue were93.8%(30/32),34.4%(11/32),respectively(P<0.01).It Wag cor - related with its clinical stages and pathological grades(P<0.05).Conclusions Hypermethylation can inacti - vate the transcription of RUNX3 and reduce its protein expression.It may be a considerable mechanism which leads to oncogenesis,metastasis of BTCC. Key words: Careinoma,Transitional Cell; Bladder Neoplagmsl; Operon; Methylation