Tumor suppressor gene methylation in tissue and urine specimens of bladder transitional cell carcinoma

Abstract

Objective To investigate abnormal promoter methylation status of death-associated protein kinase(DAPK),retinoic acid receptorβ(RARβ),Hyperplastic polyps protein(HPP1) and integrin alpha-4 precursor (ITGA4) genes,and the diagnostic value for bladder transitional cell carcinoma (BTCC).Methods Methylation-specific polymerase chain reaction (MSP) was used to detect the methylation status of DAPK,RARβ,HPP1 and ITGA4 genes in tissue and urine specimens of 112 patients with BTCC.Results The methylation rate of DAPK,RARβ,HPP1 and ITGA4 in BTCC tissue was 29.4％,91.0％,75.0％ and 48.2％ respectively,and that in urine sediment cells was 26.7％,87.5％,69.6％ and 27.6％ respectively.Aberrant methylation rate of DAPK,HPP1 and RARβ genes in BTCC was significantly different from that in the glandular cystitis (P ＜ 0.05).There was no significant difference in the methylation rate of ITGA4 between BTCC and and cystitis glandularis (P ＞ 0.05).Conclusion Aberrant methylation status of HPP1,DAPK1,and RARβ is closely related with incidence of bladder cancer. Key words: Bladder carcinoma; Urine sediment cells; Tumor suppressor gene; Methylation