Abstract
Electron Paramagnetic Resonance (EPR) spectroscopy is used to study dynamics and conformational changes in the RNA glycine riboswitch. The dynamic role of the leader-linker interaction within glycine riboswitch conserved sequences is probed through site directed spin labeling and continuous wave EPR. Inter-aptamer and aptamer-expression platform interactions are elucidated through double electron-electron resonance spectroscopy. Incorporation of spin labels is achieved through optimized ligation methodologies allowing synthetically modified RNA to be joined to larger RNA sequences. Expected folding and burial of riboswitch elements will lead to restricted motion of the spin label and, additionally, pulsed EPR experiments yield distance distribution profiles indicating conformational exchange between states in the absence and presence of glycine.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
