Abstract
<p>In this investigation, a scanning electron microscopy (SEM) method was used to examine the morphostructural changes in bacterial cells induced by antimicrobial agents. SEM-based visual approach is referred the study of bacterial cells and their physiological consequences when affected by antibiotics or antibacterial agents permitting the observation of characteristic morphological defects of cell wall, and provides valuable insights into processes involved in bacterial cell death. This experiment visualized various step-by-step techniques used in the slide preparation of bacterial cells treated with specific antimicrobial agent for analyzing the morphological alterations such as increase of cell wall roughness, cell disruption, cell swelling and lysed cell formation due to loss of intracellular material using SEM analysis when compared with untreated normal cells as a control. The SEM approach used in this visual experiment may analyze the antimicrobial effect of any commercially known or new compounds in a very conducive manner.</p><p><strong>Video Clips</strong></p><p><a href="https://youtube.com/v/WQtp1fV3Zuc">Culturing of bacteria</a> 2 min 54 sec</p><p><a href="https://youtube.com/v/U3RUXFWDgtw">Treatment of bacterial culture by antimicrobials</a> 4 min 21 sec</p><p><a href="https://youtube.com/v/AgeuJg_afeE">Sample pre-treatment</a> 4 min 23 sec</p><p><a href="https://youtube.com/v/SI7PU0Uw7Bo">Microscopic slide preparation</a> 9 min 27 sec</p><p><a href="https://youtube.com/v/ilX91i4afVM">Critical point drying and ion sputter coating of slides</a> 5 min 52 sec</p><p><a href="https://youtube.com/v/iOp9Mi_O3KE">Scanning Electron Microscopic analysis of coated slides</a> 4 min 18 sec</p>
Highlights
Bacterial contamination is increasingly becoming a cause of concern for human health (Martin and Beutin, 2011)
Prudent use of antibacterial agents using at the appropriate dosage and for the appropriate bacterial species is one of the important means of reducing the selective pressure that helps resistant organisms emerge
In the present study, scanning electron microscopy (SEM) visual experiment determined the changes in morphology of the bacteria after treatment with antibiotics/ antimicrobial agent in comparison with control cells
Summary
Bacterial contamination is increasingly becoming a cause of concern for human health (Martin and Beutin, 2011). Present study investigates visual demonstration on how to investigate the morphostructural damage in bacterial cells treated with particular antimicrobial agent (antibiotic and/or antimicrobial agent) in terms of its antimicrobial effect against pathogenic bacteria by using step-by-step visualization of scanning electron microscopic (SEM) analysis. VIDEO CLIPS Culturing of bacteria Treatment of bacterial culture by antimicrobials Sample pre-treatment Microscopic slide preparation Critical point drying and ion sputter coating of slides Scanning Electron Microscopic analysis of coated slides
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