Study the therapeutic role of Alcoholic Extract of Plantago lanceolata aganist infection with Staphylococcus saprophyticus

Aseel Jasim Mohammad

doi:10.30539/iraqijvm.v36i0e.375

Abstract

The present study was carried out to investigate the antibacterial activity of alcoholicextracts of Plantago lanceolata leafes in vitro and in vivo by inducing urinary tract infection inrats which caused by urethra administration of S.saprophyticus isolated from human andanimals(cow and sheep)These extracts showed significant effect (P<0.05) on the inhibition of the growth ofS.saprophyticus in vitro with the superiority of the concentration 200mg / ml of alcoholicextract with the mean of inhibition zone diameter 30 mm against S.saprophyticus ,while zonediameter was ( 26.5 ,21 ) mm due to the concentration 150, 100mg/ml respectively.This study included the therapeutic role of doses 150 mg/kg . B.W. of 1.5ml daily orally ofalcoholic extract dissolved in DMSO of plantago lanceolata leaves in the pathogenesis ofS.saprophyticus in rats by the urethral infection in compared with the control group (ratsinjected with S.saprophyticus without treatments).The results of histopathological changesshowed the role of Plantago lanceolata extract on the decreasing of pathological sings inbladder and kidney tissue after 14 and 21 days and gave negative results by decreasecongestion in the blood vessels of kidney hemorrhage and few infiltration of inflammatorycells in bladder , in compared with the positive control which showed acutehistopathological change.

Highlights

Many researchers preferring use of plant extract instead of antibiotics was due to attenmuation of pathogens virulence by plant extract as opposed to the direct killing of pathogenic bacteria with antibiotic as a strategy to combat infections is an interesting concept, the idea that anti-pathogenic molecules that prevents for instance the production of toxins or abolish the ability of bacteria to adapt to the host environment would give a competitive advantage to the host immune system to allow clearance of the infectious organism (1)
The uses of Plantago lanceolata orally to treat digestive and bronchial disorders and topically to treat skin disorders and eye infections are very widespread, it is used for sinus congestion, allergies, lung congestion, colitis, excess of production of mucus, diarrhea and dysentery, cystitis, nephritis and other infections (2)
The results showed the superiority of the concentration 200mg /ml in all plant extracts and this may be due to the solubility of high amount of active ingredient which inhibited the bacterial growth, these results come in agreement with that mentioned by (8)

Summary

Introduction

Many researchers preferring use of plant extract instead of antibiotics was due to attenmuation of pathogens virulence by plant extract as opposed to the direct killing of pathogenic bacteria with antibiotic as a strategy to combat infections is an interesting concept, the idea that anti-pathogenic molecules that prevents for instance the production of toxins or abolish the ability of bacteria to adapt to the host environment would give a competitive advantage to the host immune system to allow clearance of the infectious organism (1) .The uses of Plantago lanceolata orally to treat digestive and bronchial disorders and topically to treat skin disorders and eye infections are very widespread , it is used for sinus congestion, allergies, lung congestion, colitis, excess of production of mucus, diarrhea and dysentery, cystitis, nephritis and other infections (2) .The Gram-positive bacterium Staphylococcus saprophyticus can cause up to 5 to 15% of uncomplicated UTI (3).S. saprophyticus has been isolated from 7% of rectal swabs taken from carcasses of cattle and pigs. The uses of Plantago lanceolata orally to treat digestive and bronchial disorders and topically to treat skin disorders and eye infections are very widespread , it is used for sinus congestion, allergies, lung congestion, colitis, excess of production of mucus, diarrhea and dysentery, cystitis, nephritis and other infections (2). The virulence factors of S. saprophyticus include adherence to urothelial cells by means of a surface-associated protein, lipoteichoic acid; a hemagglutinin that binds to fibronectin, a hemolysin; and production of extracellular slime (5). This study was aimed to study the therapeutic role of Plantago lanceolata (alcoholic extrat) against infection with S.saprophyticus

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