Abstract
Cytochrome c in a cell, in an aqueous medium or in a non-polar environment can be both in a free state and in complex with cardiolipin. In the latter case, it is partially denatured, characterized by a specific conformation, has peroxidase activity and is an important component of proapoptotic signaling pathways [1,2,9]. Differences between native cytochrome c and the cytochrome c—cardiolipin complex are recorded by the absorption and fluorescence spectra, as well as by the pronounced peroxidase activity of the latter and chemiluminescence accompanying the free radical processes it triggers. Based on this, software was developed for automatic calculation of kinetic parameters and quantum yields, lipid peroxidation of activated chemiluminescence and visualization of model curves.
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