Study regarding the in vitro propagation in the Lilium orientale.

Abstract

The modelling of the in vitro morphogenetic process with a view to the propagation to horticultural species of commercial interest is one of the main preoccupations of the present vegetal biotechnologies. The involvement of the external phytohormones in the management of the caulogenic and rhizogenic processes represents a recognized modality for inducing and performing the in vitro propagation at various plants species [2]. The in vitro formation of minibulbs by means pf biotechnological methods, at species of the Liliaceae family, offers the advantage to obtain flower bulbs after just four years of culture instead of six – seven years necessary by means of recognized horticultural methods [13]. Consequently, this study presents both the settlement of viable aseptic cultures and the influence of cytokinins BA and TDZ in the modelling of the in vitro caulogenesis starting from explants, of the stem disk fragment type or of the flaked leaf type, sampled from flower bulbs of Lilium orientale found in the period of vegetative repose (dormancy). This study also presents the influence of auxins NAA and IBA in managing the adventive rhizogenesis at the level of the minibulbs obtained in vitro. The Lilium orientale varieties studied were selected for their commercial value as cut flowers (Lilium orientale var. stargazer and Lilium orientale var. devotion), or as pot plants (Lilium orientale var. prestige and Lilium orientale var. boulevard) – [8].