Abstract
In this paper, we present a novel wide-field fluorescence sectioning microscopy in which speckle patterns are produced on the sample for illumination. The speckle pattern is dynamically changed and a sequence of fluorescence images of the sample is recorded with a CCD camera. Due to a large variation of fluorescence intensity of the in-focus light compared to that of the out-of-focus light, special processing algorithms can be used to reconstruct the sectioned fluorescence image. We calibrated the system and studied the effect of the reconstruction algorithms on the system performance. Fluorescence sectioned images of a few biological samples were obtained. Our experiments showed that this wide-field fluorescence sectioning microscopy can be used to optically section tissue and has potential applications in the clinics.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
